Learn to Analyze RNAseq and ChIPseq Data
The NGS Integrative Genomics Core Unit (NIG) and the Core Facility Medical Biometry and Statistical Bioinformatics (MBSB) are jointly conducting an introductory course on analysing data from RNAseq and ChIPseq experiments.
This course has two main aims:
- Theoretical background along with basic statistical concepts
- for RNAseq: from data QC to differentially expressed gene lists and functional enrichment
- for ChIPseq: from data QC to motif discovery
- Hands on session using the open source Galaxy platform (web based, gwdg installation) to perform the analyses on publicly available data sourced from NCBI-SRA
Dates & time line
- Day 1: 2021-05-17, Monday, 13:00-18:00 Uhr
- raw data QC
- pre-processing of reads
- read alignment
- gene quantification
- Day 2: 2021-05-18, Tuesday, 09:00-14:00 Uhr
- differential gene expression
- visualization
- downstream analyses:
- functional enrichment using GO terms
- pathway enrichment
- biological network analysis
- Day 3: 2021-05-19, Wednesday, 13:00-18:00 Uhr
- read alignment - transcriptomic vs. genomic alignment
- ChIP QC - signal-to-noise ratio
- peak calling - TFs vs. chromatin modifications
- Day 4: 2021-05-20, Thursday, 09:00-14:00 Uhr
- peaks in replicates - to merge or not to merge
- gene annotations and feature association
- motif discovery
Instructors
- Orr Shomroni (PhD) : NGS Integrative Genomics Core Unit (NIG)
- Maren Sitte (PhD) : NGS Integrative Genomics Core Unit (NIG)
- Andreas Leha (PhD) : Core Facility for Medical Biometry and Statistical Bioinformatics
- John Wiedenhoeft (PhD) : Core Facility for Medical Biometry and Statistical Bioinformatics
Language
- English
Limited seats available (capacity of the galaxy server), preference based on first-come-first-served.
Location
online
BigBlueButton instance at the gwdg
Prerequisites
- gwdg account (guest accounts will be available if necessary)
Price
- 300 € per Person for the full course, RNAseq and ChIPseq (day 1-4)
- 200 € per Person for the RNAseq part of the course (day 1 and 2)
- 200 € per Person for the ChIPseq part of the course (day 3 and 4)
Registration
Please send an email to andreas.leha(at)med.uni-goettingen.de .